Composite

Part:BBa_K1156006:Design

Designed by: Haylee Bachman   Group: iGEM13_GeorgiaTech   (2013-09-17)


T7_lacI_RBS_NGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 265
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 877
    Illegal XhoI site found at 780
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7 is not viable in all E.coli


Source

Ligation of BBa_K1156004 and BBa_K115600

References